texas red linked streptavidin  (Vector Laboratories)

Journal: Virology

Article Title: Role of anti-gB and -gD antibodies in antibody-induced endocytosis of viral and cellular cell surface glycoproteins expressed on pseudorabies virus-infected monocytes.

doi: 10.1006/viro.1999.0132

Figure Lengend Snippet: FIG. 4. Polyclonal, but not monoclonal, antibodies against gB and gD induce cointernalization of the majority of the plasma membrane pro- teins. PrV-infected monocytes were biotinylated and incubated with a mixture of either monoclonal (1) or polyclonal (2) antibodies for 2 h at 37°C, paraformaldehyde fixed, permeabilized, and incubated with streptavidin-Texas Red and either FITC-labeled goat anti-mouse IgG or FITC-labeled rabbit anti-goat IgG. Distribution of viral glycoproteins (A)

Article Snippet: After washing in FBS, cells were incubated with either 1:30 FITC-conjugated goat antimouse antibodies (Molecular Probes) or 1:30 FITC-labeled rabbit anti-swine antibodies (DAKO A/S) together with 1:30 Texas Red-linked streptavidin (Molecular Probes) in FBS for 1 h at room temperature.

Techniques: Bioprocessing, Clinical Proteomics, Membrane, Infection, Incubation, Labeling